current research

Gallery

1cbh 6hir 2MWH

1981-1985

7. Gronenborn, A.M., Birdsall, B., Hyde, E.I., Roberts, G.C.K., Feeney, J. & Burgen, A.S.V.  Direct observation by NMR of two coexisting conformations of an enzyme-ligand complex in solution.  Nature 290, 273 (1981).

8. Gronenborn, A.M., Birdsall, B., Hyde, E.I., Roberts, G.C.K., Feeney, J. & Burgen, A.S.V.  Effects of coenzyme binding on the histidine residues of Lactobacillus casei dihydrofolate reductase. Biochemistry 20, 1717 (1981).

9. Gronenborn, A.M., Birdsall, B., Hyde, E.I., Roberts, G.C.K., Feeney, J. & Burgen, A.S.V.1H and 32P NMR characterization of two conformations of the trimethoprim-NADP+- dihydrofolate reductase complex.  Mol. Pharmacol. 20, 145 (1981).

10. Gronenborn, A.M. & Davies, R.W.  DNA binding of Lactobacillus casei dihydrofolate reductase.  J. Biol. Chem. 256, 12152 (1981).

11. Birdsall, B., Gronenborn, A.M., Clore, G.M., Roberts, G.C.K., Feeney, J. & Burgen, A.S.V.  13C-NMR evidence for three slowly interconverting conformations of the dihydrofolate reductase-NADP+ folate complex.  Biochem. Biophys. Res. Commun. 101, 1139 (1981).

12. Gronenborn, A.M., Clore, G.M. & Davies, R.W. Modulation of specific binding of Lactobacillus casei dihydrofolate reductase to DNA by folinic acid.  FEBS Lett. 133, 92 (1981).

13. Clore, G.M., Roberts, G.C.K., Gronenborn, A.M., Birdsall, B & Feeney, J. Transfer of saturation NMR studies of protein-ligand complexes: the case of three site exchange.  J. Magn. Reson. 45, 143 (1981).

14. Gronenborn, A.M., Clore, G.M., Blazy, B. & Baudras, A.  Conformational selection of syn-cAMP upon binding to the cAMP receptor protein: a 1H-NMR study. FEBS Lett. 136, 160 (1981).

15. Davies, R.W. & Gronenborn, A.M.  Molecular cloning of the gene for dihydrofolate reductase from Lactobacillus casei Gene 17, 229 (1982).

16. Clore, G.M. & Gronenborn, A.M.  Kinetic and structural studies on the intermediates formed in the reactions of 5′-adenosine monophosphate and 5′-guanosine monophosphate with cis-dichlorodiammineplatinum II using 1H and 195Pt magnetic resonance spectroscopy.  J. Am. Chem. Soc. 04, 1369 (1982).

17. Gronenborn, A.M. & Roth, K.  NMR in vivo (Part I).  Chemie in unserer Zeit 1, 1 (1982).

18. Roth, K. & Gronenborn, A.M.  NMR Tomographie (Part II) Chemie in unserer Zeit 2, 35 (1982).

19. Clore, G.M., Gronenborn, A.M. & Davies, R.W.  Theoretical aspects of specific and non-specific equilibrium binding of a protein to DNA as studied by the nitrocellulose filter binding assay: cooperative and non-cooperative binding to a one-dimensional lattice. J. Mol. Biol. 155, 447-466 (1982).

20. Clore, G.M. & Gronenborn, A.M.  Theory and applications of the transferred nuclear Overhauser effect to the study of the conformations of small ligands bound to proteins. J. Magn Reson. 48, 402 (1982).

21. Gronenborn, A.M. & Clore, G.M.  Conformation of NAD+ bound to yeast and horse liver alcohol dehydrogenase in solution: the use of the proton-proton transferred nuclear Overhauser enhancement.
J. Mol. Biol. 57, 155 (1982).

22. Takahashi, M., Gronenborn, A.M., Clore, G.M., Blazy, B. & Baudras, B.  DNA binding of cAMP receptor protein and its N-terminal core stabilizes the double helix and is modulated by cAMP.  FEBS Lett. 139, 37 (1982).

23. Gronenborn, A.M. & Clore, G.M.  Proton nuclear magnetic resonance studies on cyclic nucleotide binding to the Escherichia coli adenosine 3′,5′-phosphate receptor protein. Biochemistry 21, 4040-4048 (1982).

24. Clore, G.M. & Gronenborn, A.M.  A proton nuclear magnetic resonance study of the histidine residues of the Escherichia coli adenosine 3′,5′-phosphate receptor protein: pH titration behaviour, deuterium exchange and partial assignments. Biochemistry 21, 4048 (1982).

25. Clore, G.M. & Gronenborn, A.M.  Determination of the conformations of cyclic nucleotides bound to the N-terminal core of the cyclic AMP receptor protein of Escherichia coli by 1H-NMR. FEBS Lett. 45, 197 (1982).

26. Clore, G.M., Gronenborn, A.M., Mitchinson, C. & Green, N.M. 1H-NMR studies on nucleotide binding to the sarcoplasmic reticulum Ca2+ATPase: determination of the conformations of bound nucleotides by the measurement of proton-proton transferred nuclear Overhauser enhancements. Eur. J. Biochem. 128, 113 (1982).

27. Birdsall, B., Gronenborn, A.M., Hyde, E.I., Clore, G.M., Roberts, G.C.K., Feeney, J. & Burgen, A.S.V. 1H, 13C and 31P NMR studies of the dihydrofolate reductase-NADP+-folate complex: characterization of three coexisting conformational states. Biochemistry 21, 5831 (1982).

28. Unger, B., Clore, G.M., Gronenborn, A.M. & Hillen, W.  Specific DNA binding of the cyclic AMP receptor protein with the lac operon stabilizes double stranded DNA in the presence of cAMP.  EMBO J. 2, 289 (1983).

29. Gronenborn, A.M., Clore, G.M. & Gronenborn, B. Protection against nuclease cleavage of pBR 322 DNA by the cyclicAMP receptor protein of Escherichia coli. J. Mol. Biol.166, 93 (1983).

30. Gronenborn, A.M. & Clore, G.M. Characterization of the DNA binding region recognized by dihydrofolate reductase from Lactobacillus casei.  J. Biol. Chem. 258, 11256 (1983).

31. Clore, G.M. & Gronenborn, A.M.  Theory of the time dependent transferred nuclear Overhauser effect: application to the structural analysis of ligand-protein complexes in solution. J. Magn. Reson. 53, 423 (1983).

32. Clore, G.M., Kimber, B.J. & Gronenborn, A.M. The 1-1 hard pulse: a novel, simple and effective method of water resonance suppression in FT-1H-NMR. J. Magn. Reson. 54, 170 (1983).

33. Martin, S.R., Gronenborn, A.M. & Clore, G.M.  Specific DNA binding of the cAMP receptor protein to a synthetic oligodeoxyribonucleotide: a circular dichroism study. FEBS Lett. 159, 102 (1983).

34. Gronenborn, A.M., Kimber, B.J., Clore, G.M. & McLaughlin, L.W. A nuclear magnetic resonance study of the ribotrinucleoside diphosphate UpUpC. Nucl. Magn. Res. 11, 5691 (1983).

35. Clore, G.M., Gronenborn, A.M. & Davies, R.W. Cooperative non-specific DNA binding of the N-terminal core of the cyclic AMP receptor protein of Escherichia coli and its modulation by cyclic AMP.  FEBS Lett. 164, 57 (1983).

36. Clore, G.M. & Gronenborn, A.M. Sequence dependent structural variations in two right handed alternating pyrimidine-purine DNA oligomers in solution determined by nuclear Overhauser enhancement measurements. EMBO J. 2, 2109 (1983).

37. Gronenborn, A.M., Clore, G.M., Jones, M.B. & Jiricny, J.  A nuclear Overhauser enhancement study on the imino proton resonances of a DNA pentadecamer comprising the specific target site of the cyclic AMP receptor protein in the ara BAD operon. FEBS Lett. 165, 216 (1984).

38. Clore, G.M., Gronenborn, A.M., Birdsall, B.M., Feeney, J. & Roberts, G.C.K. 19F-NMR studies of 3′,5′- difluoromethotrexate binding to Lactobacillus casei dihydrofoate reductase: molecular motion and coenzyme induced conformational changes. Biochem. J. 217, 659 (1984).

39. Gronenborn, A.M., Papadopoulos, P. & Clore, G.M. Immunochemical evidence for extensive ligand induced conformational changes in Lactobacillus casei dihydrofolate reductase. J. Biol. Chem. 259, 1082 (1984).

40. Antonjuk, D.J., Birdsall, B.M., Chang, H.T.A., Clore, G.M., Feeney, J., Gronenborn, A.M., Roberts, G.C.K. & Tran, T.Q.  A 1H-NMR study of the role of the glutamate moiety in the binding of methotrexate to Lactobacillus casei dihydrofolate reductase.  Bri J. Pharmacol. 81, 309 (1984).

41. Gronenborn, A.M., Clore, G.M. & Jeffery, J.  An unusual conformation of NAD+ bound to sorbitol dehydrogenase: a time dependent transferred nuclear Overhauser effect study. J. Mol. Biol. 172, 559 (1984).

42. Clore, G.M., Gronenborn, A.M. & McLaughlin, L.W. The structure of the ribotrinucleoside diphosphate codon UpUpC bound to tRNAPhe from yeast: a time dependent transferred nuclear Overhauser effect study. J. Mol. Biol. 174, 163 (1984).

43. Clore, G.M. & Gronenborn, A.M.  A Nuclear Overhauser enhancement study of the solution structure of a double stranded DNA undecamer comprising a portion of the specific target site for the cyclic AMP receptor protein in the gal operon: sequential resonance assignment. Eur. J. Biochem. 141, 119 (1984).

44. Gronenborn, A.M., Clore, G.M. & Kimber, B.J. An investigation into the solution structures of two self-complementary DNA oligonucleotides 5’d-CGTACG and 5’d-ACGCGCGT by means of nuclear Overhauser enhancement measurements. Biochem. J. 221, 723 (1984).

45. Clore, G.M., Gronenborn, A.M., Piper, E.A., McLaughlin, L.W., Graeser, E. & van Boom, J.H.  The solution structure of a RNA pentadecamer comprising the anticodon loop and stem of yeast tRNAPhe: a 500 MHz 1H-NMR study. Biochem. J. 221, 737 (1984).

46. Birdsall, B.M., Bevan, A.W., Pascual, C., Roberts, G.C.K., Feeney, J., Gronenborn, A.M. & Clore, G.M.  Multinuclear NMR characterization of two coexisting conformational states of the Lactobacillus casei dihydrofolate reductase-trimethoprim-NADP+ complex. Biochemistry 23, 4733 (1984).

47. Gronenborn, A.M., Clore, G.M., Brunori, M., Giardina, B., Falcioni, G. & Perutz, M.F. Stereochemistry of ATP and GTP bound to fish haemoglobins: a transferred nuclear Overhauser enhancement, 31P-NMR, oxygen equilibrium and molecular modelling study.  J. Mol. Biol. 178, 731 (1984).

48. Clore, G.M. & Gronenborn, A.M. An investigation into the solution structure of the single stranded DNA undecamer 5’d- AAGTGTGATAT by means of nuclear Overhauser enhancement measurements.  Eur. Biophys. J. 11, 95 (1984).

49. Clore, G.M. & Gronenborn, A.M.  Internal mobility in a double stranded B DNA hexamer and undecamer: a time dependent proton-proton nuclear Overhauser enhancement study. FEBS Lett. 172, 219 (1984).

50. Chung, S.H., Johnson, M.S. & Gronenborn, A.M.  L-cycloserine: a potent anti-convulsant.  Epilepsy 25, 353 (1984).

51. Clore, G.M. & Gronenborn, A.M.  Interproton distance measurements in solution for a double stranded DNA undecamer comprising a portion of the specific target site for the cyclic AMP receptor protein in the gal operon: a nuclear Overhauser enhancement study. FEBS Lett. 175, 117 (1984).

52. Gronenborn, A.M., Clore, G.M., McLaughlin, L.W., Graeser, E., Lorber, B. & Giege, R. Yeast tRNAAsp – Codon and wobble codon-anticodon interactions: a transferred nuclear Overhauser enhancement study.  Eur. J. Biochem. 145, 359 (1984).

53. Gronenborn, A.M., Clore, G.M., Hobbs, L. & Jeffery, J.  Glucose-6-phosphate d    ehydrogenase: a transferred nuclear Overhauser effect study of NADP+ conformations in enzyme-coenzyme binary complexes. Eur. J. Biochem. 145, 365 (1984).

54. Clore, G.M., Lauble, H., Frenkiel, T.A. & Gronenborn, A.M.  A two-dimensional NMR study of the solution structure of a DNA dodecamer comprising the consensus sequence for the specific DNA binding sites of the glucocorticoid receptor protein. Eur. J. Biochem. 145, 629 (1984).

55. Gronenborn, A.M., Nermut, M.V., Eason, P. & Clore, G.M.  Visualization of cAMP receptor protein induced DNA kinking by electron microscopy. J. Mol. Biol. 179, 751 (1984).

56. Clore, G.M. & Gronenborn, A.M.  Assessment of errors involved in the determination of interproton distance ratios and distances by means of one- and two-dimensional NOE measurements.     J. Magn. Reson. 61, 158 (1985).

57. Clore, G.M. & Gronenborn, A.M.  Probing the three-dimensional structures of DNA and RNA oligonucleotides in solution by nuclear Overhauser enhancement measurements. FEBS Lett. 179, 187 (1985).

58. Gronenborn, A.M. & Clore, G.M.  Investigations into the solution structures of short nucleic acid fragments by means of nuclear Overhauser enhancement measurements. Progr. Nucl. Magn. Reson. Spectroscopy 17, 1 (1985).

59. Andrews, J., Clore, G.M., Davies, R.W., Gronenborn, A.M., Gronenborn, B., Kalderon, D., Papadopoulos, P.C., Schafer, S., Sims, P.F.G. & Stancombe, R.  Nucleotide sequence of the dihydrofolate reductase gene of methotrexate resistant Lactobacillus casei.  Gene 35, 217 (1985).

60. Perutz, M.F., Gronenborn, A.M., Clore, G.M., Hogg, J.H. & Shi, T.  The pKa values of two histidine residues in human haemoglobin, the Bohr effect and the dipole moment of -helices. J. Mol. Biol. 183, 491-498 (1985).

61. Perutz, M.F., Gronenborn, A.M., Clore, G.M., Shi, T. & Craescu, C.T.  Comparison of histidine proton magnetic resonances of human carbonmonoxy haemoglobin in different buffers. J. Mol. Biol. 186, 471 (1985).

62. Clore, G.M. & Gronenborn, A.M.  The solution structure of a B-DNA undecamer comprising a portion of the specific target site for the cyclic AMP receptor protein in the gal operon: refinement on the basis of interproton distance data. EMBO J. 4, 829 (1985).

63. Clore, G.M., Gronenborn, A.M., Moss, D.S. & Tickle, I.J.  Refinement of the solution structure of the B-DNA hexamer 5’d(CGTACG)2 on the basis of interproton distance data. J. Mol. Biol. 185, 219 (1985).

64. Clore, G.M., Gronenborn, A.M. & McLaughlin, L.W.  The structure of the double stranded RNA pentamer 5′(CACAG).5′(CUGUG) by nuclear Overhauser enhancement measurements: interproton distance determination and structure refinement on the basis of X-ray coordinates.  Eur. J. Biochem. 151, 153 (1985).

65. Clore, G.M., Gronenborn, A.M., Brunger, A.T. & Karplus, M. The solution conformation of a heptadecapeptide comprising the DNA binding helix F of the cyclic AMP receptor protein of Escherichia coli: combined use of 1H-nuclear magnetic resonance and restrained molecular dynamics.   J.. Mol. Biol. 186, 435 (1985).